GLOBAL® TOTAL® LP FOR FERTILIZATION

Human oocyte culture and fertilization.

 

Reference:

H5TF-020, 20 ml.

H5TF-050, 50 ml.

H5FT-100, 100 ml.

 

Store at 2-8°C and protected from light. Ten (10) weeks from the date of manufacture. For best results, use within four (4) weeks.

 

Assay (performed for each batch)	Specification
pH (with 5% CO2)	7.2-7.4
Osmolality	260-270 mOsM
Endotoxin (LAL)	< 0.5 EU/ml
Sterility Test (bacterial and fungal screen, SAL 10–3)	PASS
1-cell Mouse Embryo Assay (% expanded blastocysts at 96 h of culture)	> 80%
Special Note on the CO2 Concentration in the Incubator: In most cases, a 5-7% concentration of CO2 in the incubator will produce a pH of 7.2 to 7.4 in global® total® LP for Fertilization. However, the exact concentration of CO2 required to produce the optimum pH of approximately 7.30 (7.27-7.33) depends on several factors, including the physical characteristics of incubator and the altitude. Consequently, we strongly recommend that each laboratory determine and use the concentration of CO2 that is required to produce a pH of 7.30 in global® total® LP for Fertilization.