PureSperm 100 is a sterile colloidal silica suspension in an isotonic salt solution. It is optimized for the preparation of density gradients used to separate and purify human sperm from semen for use in Assisted Reproduction Technologies (ART). This system effectively separates normal sperm from lymphocytes, epithelial cells, abnormal, immature and senescent sperm, cell debris, bacteria and seminal fluid.
Features:
- Increases sperm survival time.
- Removes sources of ROS.
- Removes bacteria.
- Removes sperm with abnormal DNA.
- Improves cryo-survival.
- Improves yield and recovery compared to swim-up techniques.
Components
– Silane-coated silica
– Calcium chloride
– Potassium chloride
– Sodium chloride
– Purified water
– HEPES
– EDTA
– Glucose
Performance Characteristics
- pH: 7.4-7.8.
- Osmolality (mOsm/kg H2O): 300-310.
- Endotoxin transfer during treatment: <1.0 EU/mL.
- Sperm survival 18 hours after density gradient separation: >70%.