PureSperm 40/80/90

All the advantages of a PureSperm 100 gradient, plus ready-diluted to save preparation time. PureSperm 40, PureSperm 80 in both 100mL & 20mL and PureSperm 90 in 100mL, are formulated to minimize sudden pH and osmolarity changes during the transition of sperm from the semen sample to the fertilization medium. This helps to avoid premature hyperactivation and improves fertilization potential.

Features:

- PureSperm 40, PureSperm 80 and PureSperm 90 are formulated to minimize sudden pH and osmolarity changes during the transition of sperm from a semen sample to the fertilization medium. This helps to avoid premature hyperactivation and improves fertilization potential.

- PureSperm 40, PureSperm 80 and PureSperm 90 maintain pH between 7.4 – 7.8 at room temperature and at 37°C, providing suitable conditions for good sperm motility, survival time, and fertilization potential.

- The preparation contains glucose as a usable energy substrate for normal cell metabolism and sperm function.

- The gradient enables motile sperm to be separated from extraneous cells and seminal plasma. In addition, damage to sperm DNA and structural molecules caused by reactive oxygen species (ROS) is minimized by the effective removal of immature sperm and lymphocytes. Bacteria, epithelial cells, and cell debris are also separated from the motile sperm, which can be difficult to achieve in polysucrose-based gradient preparations.

Components

– Silane-coated silica
– Potassium chloride
– Sodium chloride
– Purified water
– HEPES
– EDTA
– Glucose
– Sodium pyruvate
– Calcium chloride

Performance Characteristics

- pH: 7.4 – 7.8.

- Osmolality (mOsm/kg H2O) : 300 – 310.

- Endotoxin transfer during treatment : <1.0 EU/mL.

- Sperm survival 18 hours after density gradient separation: >70%.

- Contents are tested by human sperm survival.

- Bottles and stoppers are M.E.A. tested.

Cant.