Reference:
SIS100 |
Sil-Select Stock - 100mL |
SIS500 |
Sil-Select Stock - 500mL |
SIS100G |
Sil-Select Stock with gentamicin - 100mL |
Sperm preparation media - stock solution.
Sil-Select Stock™ is our home made stock solution for preparing density gradient system for semen preparation. Sil-Select Stock™ is an isotonic solution of silane-coated sicila particles in a chemically defined, balanced salts solution with HEPES (pH buffer). Sil-Select Stock can be used in combination with IUI, IVF and ICSI. The density of Sil-Select Stock™ is approximately 1.12g/ml.
Extensive testing during the design of the product has shown it to be non-cytotoxic and non-irritant with a non-significant allergenic potential.
Sil-Select Stock™ is manufactured from raw colloidal silica, so we have full control over each step in the production process. We have developed a very accurate purification system which results in a very clean product with endotoxin levels below 0.5EU/ml.
Component |
Benefit |
HEPES (18 mM) |
HEPES stabilizes the pH under air (Clark and Swain 2014), therefore CO2 incubation is not required.
|
Earle’s Balanced Salt Solution (EBSS) |
The composition of the salt solution results in an optimum pH and osmolality environment for sperm cells.
|
Silane coated colloidal silica |
Density gradient media have been optimized for maximum efficiency in the selection of spermatozoa for use in ART. Their density is directly linked with their functionality and depends on the % silanized colloidal silica particles added in the media.
|